p53 dependent fluc reporter plasmid pg13 luc Search Results


p53 dependent fluc reporter plasmid pg13 luc  (Addgene inc)
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Addgene inc p53 dependent fluc reporter plasmid pg13 luc
tRNA GluV13 rescues endogenous nonsense mutations in TP53 . ( A ) Representative western blots of cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Lysates were probed for <t>p53</t> and CBP80 (loading control) 48 h after transfection. Bands corresponding to full-length (FL p53) and truncated (TR p53) are indicated. ( B ) p53-dependent luciferase activity in cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Data are displayed as the fold change in luciferase activity relative to cells transfected with an empty vector and reported as mean ± SD, n = 3. * P < 0.05, *** P < 0.0005, ns = not significant, unpaired Student’s t -test.
P53 Dependent Fluc Reporter Plasmid Pg13 Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p53+dependent+fluc+reporter+plasmid+pg13+luc/PG13-Luc+(p53)+(Plasmid+%2316251)/pmc12204705-163-21-31
Average 93 stars, based on 1 article reviews
p53 dependent fluc reporter plasmid pg13 luc - by Bioz Stars, 2026-10
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tRNA GluV13 rescues endogenous nonsense mutations in TP53 . ( A ) Representative western blots of cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Lysates were probed for p53 and CBP80 (loading control) 48 h after transfection. Bands corresponding to full-length (FL p53) and truncated (TR p53) are indicated. ( B ) p53-dependent luciferase activity in cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Data are displayed as the fold change in luciferase activity relative to cells transfected with an empty vector and reported as mean ± SD, n = 3. * P < 0.05, *** P < 0.0005, ns = not significant, unpaired Student’s t -test.

Journal: Nucleic Acids Research

Article Title: An engineered glutamic acid tRNA for efficient suppression of pathogenic nonsense mutations

doi: 10.1093/nar/gkaf532

Figure Lengend Snippet: tRNA GluV13 rescues endogenous nonsense mutations in TP53 . ( A ) Representative western blots of cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Lysates were probed for p53 and CBP80 (loading control) 48 h after transfection. Bands corresponding to full-length (FL p53) and truncated (TR p53) are indicated. ( B ) p53-dependent luciferase activity in cells transfected with an empty vector or a plasmid containing three copies of tRNA Pyl (control tRNA) or tRNA GluV13 CUA . Data are displayed as the fold change in luciferase activity relative to cells transfected with an empty vector and reported as mean ± SD, n = 3. * P < 0.05, *** P < 0.0005, ns = not significant, unpaired Student’s t -test.

Article Snippet: The following day, cells were transfected with 50 ng of the indicated sup-tRNA plasmid (or an empty vector), 50 ng of p53-dependent Fluc reporter plasmid PG13-luc (a gift from Bert Vogelstein; Addgene Plasmid # 16442; http://n2t.net/addgene:16442; RRID:ADDGENE_16442 ), and 15 ng of pLX313- Renilla .

Techniques: Western Blot, Transfection, Plasmid Preparation, Control, Luciferase, Activity Assay